Public health assessment of Salmonella enterica serovar enteritidis inactivated-vaccine treatment in layer flocks.

Salmonella enterica Serovar Enteritidis (SE) Infection in Chickens and Its Public-Health-Risk Control Using an SE Vaccine in Layer Flocks

Occurrence of multidrug-resistant Salmonella enterica serovar Enteritidis isolates from poultry in Iran

Salmonella enterica is recognized as one of the major food-borne pathogens with more than 2,500 serotypesworldwide. The present study addresses antimicrobial resistance of Salmonella enterica serovar Enteritidisisolates in Iran. A collection of 151 Salmonella spp. isolates collected from poultry were serotyped toidentify Salmonella Enteritidis. Sixty-one Salmonella Enteritidis were subsequently...

Salmonella enterica serovar Enteritidis live vaccine strain in the reproductive organs of laying goose after subcutaneous vaccination

Serovar-specific real-time PCR for Salmonella enterica serovar Enteritidis (S. Enteritidis) was conductedto detect the genomic DNA of S. Enteritidis from laying goose after subcutaneous vaccination at differenttime points. Indirect fluorescent antibody (IFA) technique and immunohistochemical localization wereemployed to validate the results. The results showed that S. Enteritidis was consistent...

An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments

In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treat...

Immunoprotectivity of Salmonella enterica serovar Enteritidis virulence protein, InvH, against Salmonella typhi

Objective(s):Typhoid fever is a dreadful disease of a major threat to public health in developing countries. Vaccination with bacterial immunodominant components such as surface proteins may prove as a potent alternative to live attenuated vaccines. InvH, an important part of needle complex in type three secretion system (TTSS) plays important role in efficient bacterial adherence and entry int...